iq5 real‐time polymerase chain reaction machine (bio‐rad) (Bio-Rad)
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iq5 real‐time polymerase chain reaction machine (bio‐rad)
Iq5 Real‐Time Polymerase Chain Reaction Machine (Bio‐Rad), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iq5+real%E2%80%90time+polymerase+chain+reaction+machine+(bio%E2%80%90rad)/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc06512105-58-8-14
Average 90 stars, based on 1 article reviews
Iq5 Real‐Time Polymerase Chain Reaction Machine (Bio‐Rad), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iq5+real%E2%80%90time+polymerase+chain+reaction+machine+(bio%E2%80%90rad)/CFX96+and+CFX384+Real-Time+PCR+Detection+Systems+Firmware+Update/pmc06512105-58-8-14
Average 90 stars, based on 1 article reviews
iq5 real‐time polymerase chain reaction machine (bio‐rad) - by Bioz Stars,
2026-09
90/100 stars
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Article Snippet: CTR and HFD male mice were sacrificed between 9 AM and 11 AM by isoflurane inhalation, pituitaries dissected, and blood obtained from the inferior vena cava. mRNA levels Pituitaries were dissected, total RNA extracted using microRNA kit (Ambion) and reverse transcribed using Superscript III (Invitrogen, CA). qPCR was performed using an iQ SYBR Green supermix and an Article Title: Pretreatment with platelet-rich plasma protects against ischemia–reperfusion induced flap injury by deactivating the JAK/STAT pathway in mice Article Snippet: Total RNAs (5 μg) were extracted from each sample using Trizol reagent (Invitrogen, Waltham, MA, USA) and reverse transcribed into cDNA in a 20-μl reaction mixture using an RT reagent kit (Takara, Shiga, Japan). qRT-PCR was performed in a Article Title: The acyl-acyl carrier protein thioesterases GmFATA1 and GmFATA2 are essential for fatty acid accumulation and growth in soybean. Article Snippet: Wenying Liao, Runze Guo, Kun Qian, Wanxuan Shi, James Whelan and Huixia Shou State Key Lab of Plant Environmental Resilience, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, P. R. China, The Provincial International Science and Technology Cooperation Base on Engineering Biology, International Campus of Zhejiang University, Haining, Zhejiang 314400, China, and Hainan Institute, Zhejiang University, Sanya, Hainan 572025, China Article Title: Antibodies specific to glycosylated PD-L1 and methods of use thereof Article Snippet: To measure the expression of mRNA, cDNA was synthesized from 1 μg purified total RNA by Superscript III First-Strand cDNA synthesis system using random hexamers (Life Technologies) according to the manufacturer's instructions. qPCR was performed using a Article Title: PD-1 blockade in combination with dasatinib potentiates induction of anti-acute lymphocytic leukemia immunity. Article Snippet: The sequences of quantitative PCR (qPCR) primers are listed in the online supplemental table 3. qPCR was performed using Reverse Transcription:Article Title: PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1 Article Snippet: Total RNA was extracted from mouse liver tissues using Trizol (Invitrogen, Waltham, USA) and Ultrapure RNA kit (CWBIO, Taizhou, China), according to the manufacturers’ protocols. .. 1 μg of total RNA was reverse-transcribed using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO, FSQ-301, Osaka, Japan). qRT-PCR was performed on a Real-time Polymerase Chain Reaction:Article Title: PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1 Article Snippet: Total RNA was extracted from mouse liver tissues using Trizol (Invitrogen, Waltham, USA) and Ultrapure RNA kit (CWBIO, Taizhou, China), according to the manufacturers’ protocols. .. 1 μg of total RNA was reverse-transcribed using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO, FSQ-301, Osaka, Japan). qRT-PCR was performed on a Article Title: TEM1/endosialin/CD248 promotes pathologic scarring and TGF-β activity through its receptor stability in dermal fibroblasts Article Snippet: Two μg total RNA was reversely transcribed into complementary DNA using 200 ng oligo (dT) 15 primer, 1 mM deoxynucleotides, 5X reverse transcriptase buffer, 20 units RNase inhibitor, and 200 units M-MLV reverse transcriptase, purchased from Thermo Fisher Scientific. .. Then, 20 ng of complementary DNA was amplified and detected by Quantitative RT-PCR:Article Title: PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1 Article Snippet: Total RNA was extracted from mouse liver tissues using Trizol (Invitrogen, Waltham, USA) and Ultrapure RNA kit (CWBIO, Taizhou, China), according to the manufacturers’ protocols. .. 1 μg of total RNA was reverse-transcribed using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO, FSQ-301, Osaka, Japan). qRT-PCR was performed on a SYBR Green Assay:Article Title: PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1 Article Snippet: Total RNA was extracted from mouse liver tissues using Trizol (Invitrogen, Waltham, USA) and Ultrapure RNA kit (CWBIO, Taizhou, China), according to the manufacturers’ protocols. .. 1 μg of total RNA was reverse-transcribed using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO, FSQ-301, Osaka, Japan). qRT-PCR was performed on a Polymerase Chain Reaction:Article Title: PTEN deficiency potentiates HBV-associated liver cancer development through augmented GP73/GOLM1 Article Snippet: Total RNA was extracted from mouse liver tissues using Trizol (Invitrogen, Waltham, USA) and Ultrapure RNA kit (CWBIO, Taizhou, China), according to the manufacturers’ protocols. .. 1 μg of total RNA was reverse-transcribed using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO, FSQ-301, Osaka, Japan). qRT-PCR was performed on a Amplification:Article Title: TEM1/endosialin/CD248 promotes pathologic scarring and TGF-β activity through its receptor stability in dermal fibroblasts Article Snippet: Two μg total RNA was reversely transcribed into complementary DNA using 200 ng oligo (dT) 15 primer, 1 mM deoxynucleotides, 5X reverse transcriptase buffer, 20 units RNase inhibitor, and 200 units M-MLV reverse transcriptase, purchased from Thermo Fisher Scientific. .. Then, 20 ng of complementary DNA was amplified and detected by |